Superresolution Shadow Imaging (SuShI) is a microscopy technique, developed to image the intercellular space and outer cell morphology. This is done by bathing tissue or cells in a solution filled with fluorophores that are not membrane permeable. These fluorophores are then imaged with STED to get a picture of everything that is not tissue. By inverting the resulting negative image, it is possible to analyze the morphology of the tissue.
